We found that the mouse Na v 1.8 gene contains two putative conserved NRSE sites, a forward-oriented sequence within the 5′-untranslated region (NRSE-1) and a reverse-oriented sequence within intron 10 (NRSE-2) (Fig. 1 B, C).
Jan 11, 2016 for a human, a genomic reference sequence does not contain any useful information (a coding DNA reference sequence does); a gene can be
2007-06-07 In non-neuronal cells, neuron-restrictive silencer factor (NSRF) actively represses gene transcription via a sequence-specific DNA motif known as the neuron-restrictive silencer element (NRSE). This DNA motif has been identified in many genes that are specific markers … 2015-05-14 2009-04-02 In addition, we inserted the tandem NRSE sequence or mutant NRSE into pGL3‐control vector . We then performed assays to determine whether the CART NRSE encodes the cis‐regulatory function. Relative to the control vector, transcriptional activity of the CART NRSE construct was significantly inhibited in … Moreover, because NRSE/RE1 has a considerable sequence variability within each species, plasmids containing NRSE/RE1 sites might be more useful than protein‐based competitors or dominant‐negative REST constructs in specifically targeting groups of REST‐regulated genes as a therapeutic approach in certain neurological diseases.
A homology search for NRSE-like sequences identified NRSE-like sequences within the genomic sequence corresponding to intron 8 and exon 9 of the human genes but not in those of other species ( Supplemental Table 2 ). 2006-07-09 · Using comparative sequence analysis, here we report the identification of 895 sites (NRSE) as the putative targets of REST. A set of the identified NRSE sites is present in the vicinity of the miRNA genes that are specifically expressed in brain-related tissues, suggesting the transcriptional regulation of these miRNAs by REST. RNA-containing NRSE sequence forms double-stranded Neuronal Lineage Induction by the NRSE dsRNA RNA (dsRNA) in lengths of about 20 bp, and that the To determine the function of the NRSE dsRNA, we ex- NRSE dsRNA activates expression of NRSE / RE1 -con- pressed them in HCN-A94 cells. So, a model is emerging whereby cells that are to become neurons activate the transcription of genes that contain the NRSE/RE1 sequence. These cells might then generate non-coding NRSE/RE1 dsRNAs Schoenherr and Anderson (1995) showed that NRSF bound the NRSE DNA sequence in the 5-prime region of SCG10.
As shown in the inset of Fig. 1 , the NRSE-like sequence identified within the intron of the HCN4 gene is highly conserved among several species and is homologous to the consensus NRSE, suggesting that it might mediate an important regulatory function in the HCN4 transcription. 2019-06-03 · Home Support Difference Between Differential, RSE, and NRSE Terminal Configurations This content is not available in your preferred language. The content is shown in another available language.
a membrane targeting sequence, and whose promoter region contained both a neuron-restrictive silencing element (NRSE) and a cAMP-response element
We predicted that the Repressor Element Silencing Transcription Factor/Neuronal Restrictive Silencer Factor (REST/NRSF) regulates expression of ICP22 and ICP4. 2000-07-14 · The ∼21 base pair RE-1/NRSE sequence has been found in a number of neuron specific genes including the type II sodium channel , the SCG10 gene , synapsin I , NMDA receptor , and the cholinergic gene locus to name but a few. The NRSE sequence of pEGFP‐N2‐NRSE4× corresponds to the NRSE site of the human synapsin promoter and differs from the NRSE consensus sequence [] by one less conserved nucleotide (TTCAGCACCGCGGACAGTGCCTT) and to the NRSE dsRNA sequence [] by one further nucleotide outside the 17 nucleotides core sequence.
The repressor element-1/neuron-restrictive silencing element (RE-1/NRSE) mediates The RE-1/NRSE sequence found in the first intron of the CRH gene
This plasmid is available through Addgene. 2008-10-01 NRSE-containing sequence (positions -233 to -209) was placed upstream ofa minimal c-fos promoter fused to the luciferase structure gene (pNR-fLUC). Transient transfec-tion assays ofthis construct showed that a single copy of synapsin I NRSE repressed the fos promoter activity by 2-foldinHeLacells(Fig. 2). Additionofonemorecopyofthe 2000-09-01 In the NIH3T3 cell line, the RE-1/NRSE sequence leads to repression of reporter-gene activity, whereas introduction of exogenous REST4 leads to de-repression.
sequence defined by this dsRNA is NRSE/RE1, which small, noncoding RNAs from adult hippocampal neural is recognized by NRSF/REST, known primarily as a stem cells during lineage-specific differentiation. To negative transcriptional regulator that restricts neu-identify possible target nucleic acids, public database
In RNA immunoprecipitation and gel-shift assays, NRSF specifically interacted with the NRSE sequence of MOR mRNA. When MOR and NRSF genes were coexpressed, the specific ligand-binding activity of MOR was increased in neuroblastoma NMB cells, but decreased in PC12 cells result from its localization. The NRSE sequence from the human BNP promoter or its mutated sequence was inserted, in tandem, into the pGL4.10-TK-luc reporter plasmid (Fig. 4A ). As shown in Fig. 4B, each reporter containing the intact NRSE sequence (left panel) exhibited less luciferase activity than those with the mutated NRSE sequences (right panel). 2004-05-01
In addition to the influence on the ANP gene, the NRSE sequence regulates other cardiac embryonic genes, such as BNP, skeletal α-actin, and Na, K – ATPase α3 subunit.
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The NRSE sequence consisting of 5′-TTCAGCACCACGGAGAGTGCC-3′ from the SCG10 gene 8 was used in this study Stéphanie De Gois, Leı̈la Houhou, Yoshio Oda, Marilys Corbex, Fabrice Pajak, Etienne Thévenot, Guilan Vodjdani, Jacques Mallet, Sylvie Berrard 2007-02-02 · The computer search revealed that an NRSE-like sequence was located ∼2 kb upstream of exon 2 of the HCN4 gene (Fig. 1, upper panel).
Depending on the number of qualified applicants, meeting the minimum criteria for admission may not be sufficient to be admitted to the professional nursing sequence in the desired semester. NRSE sequences were effective in restricting expression of bipartite Gal4-based ‘driver’ transgenes within the context of an enhancer trap and when associated with a defined promoter and enhancer. NRSE is a regulatory sequence that is present in several neuronal genes (8) and that was, up to now, thought to silence neuronal gene transcription in nonneuronal cells (6, 7, 9 – 14).
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NRSE sequences were effective in restricting expression of bipartite Gal4-based ‘driver’ transgenes within the context of an enhancer trap and when associated with a defined promoter and enhancer. NRSE is a regulatory sequence that is present in several neuronal genes (8) and that was, up to now, thought to silence neuronal gene transcription in nonneuronal cells (6, 7, 9 – 14). Region 1 contains a 21-bp sequence homologous to the neuron-restrictive silencer element (NRSE, Ref. 26) of the SCG10 gene or repressor element 1 (RE1, Ref. 27) of the type II sodium channel gene. In non-neuronal cells, neuron-restrictive silencer factor (NSRF) actively represses gene transcription via a sequence-specific DNA motif known as the neuron-restrictive silencer element (NRSE). This DNA motif has been identified in many genes that are specific markers for cells of neuronal and neuroendocrine lineage.